Template Switch Oligo
Template Switch Oligo - The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. We recommend a tso with rgrgrg at the 3′ end. The tso adds a common 5'. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor.
The tso adds a common 5'. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. We recommend a tso with rgrgrg at the 3′ end.
Template Switch Oligo
Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. In conjunction with a template switching oligo (tso), cdna is synthesized with a known.
Template Switch Oligo
In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. We recommend a tso with rgrgrg.
Template Switch Oligo Printable Word Searches
We recommend a tso with rgrgrg at the 3′ end. The tso adds a common 5'. The resulting cdna can be amplified by pcr or serve as. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. However, if a large fraction of the library contains a portion.
Template Switch Oligo Printable Word Searches
We recommend a tso with rgrgrg at the 3′ end. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached.
Template Switch Oligo
A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. We recommend a tso with rgrgrg at the 3′ end. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. However, if a large fraction of the library contains a portion. Template switching oligonucleotide and capturing oligo.
Template Switch Oligo - In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. The resulting cdna can be amplified by pcr or serve as. The tso adds a common 5'. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end.
In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. However, if a large fraction of the library contains a portion. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor.
The Tso Adds A Common 5'.
Use this set of oligonucleotides for template switching cdna synthesis and preamplification. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains a portion.
(Step 2) Mmlv Reverse Transcriptase Adds Deoxycytosines To The Cdna 3' End.
We recommend a tso with rgrgrg at the 3′ end. The resulting cdna can be amplified by pcr or serve as. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end.